Composite

Part:BBa_K3722009:Design

Designed by: Gao Zhan   Group: iGEM21_NWU-CHINA-A   (2021-09-30)


T7_Promoter-RBS-SttH-T7_Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1635
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 268
    Illegal NgoMIV site found at 526
    Illegal AgeI site found at 755
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We didn't sure whethere need to add 6*His in this squence ,so we did a series experiments to ecplore protein expression level.At last we found a Fre lable is more suitable than His.


Source

Synthesis

References